Journal: Nucleic Acids Research

Article Title: A novel dual histone mark reader ZCWPW2 regulates meiotic recombination through lactylation and transcriptional regulation in humans and mice

doi: 10.1093/nar/gkag049

Figure Lengend Snippet: ZCWPW2 shows enhanced enrichment at sites co-marked by H3K4me3 and H3K36me3 in the presence of PRDM9 and is found in complex with ZCWPW1 at these sites. ( A ) Schematic overview of CUT&Tag profiling of ZCWPW2 genomic binding sites in HEK293T cells transfected with Flag- ZCWPW2 (W2) alone or co-transfected with Myc- PRDM9 B . ( B ) Pie charts showing the genomic annotation of ZCWPW2 (W2) binding peaks in HEK293T cells transfected with Flag- ZCWPW2 alone (left) or co-transfected with Myc- PRDM9 B (right). ( C ) Bar graph showing the percentage of the top 10 000 ZCWPW2 (W2) or ZCWPW1 (W1) peaks overlapping with PRDM9-binding sites in the presence or absence of PRDM9. ( D ) Line plot showing ZCWPW2 (W2) binding signal centered around PRDM9 binding motifs (±3 kb) in HEK293T cells expressing Flag-ZCWPW2 with or without Myc-PRDM9. ( E ) Heatmaps and average signal plots showing ZCWPW2 (W2) enrichment centered on PRDM9-binding peaks (±2 kb) in HEK293T cells transfected with Flag- ZCWPW2 alone, co-transfected with Myc- PRDM9 B , or IgG control. ( F ) Venn diagram showing the overlap among ZCWPW2 (W2), ZCWPW1 (W1), and PRDM9 peaks in HEK293T cells co-expressing Myc-PRDM9. ( G ) Genome tracks snapshot showing representative genomic regions with binding profiles of ZCWPW2 (W2), ZCWPW1 (W1), and PRDM9 in HEK293T cells. ( H ) Genome track snapshots showing ZCWPW2 (W2) signals (green) in HEK293T cells co-expressing Flag-ZCWPW2 and Myc-PRDM9, and ZCWPW2 (W2) signals (blue) in cells expressing Flag-ZCWPW2 alone, together with tracks for PRDM9-induced H3K4me3 (pink) and H3K36me3 (orange). ( I ) ZCWPW2 peaks at H3K4me3/H3K36me3 sites in HEK293T cells co-expressing Flag-ZCWPW2 and Myc-PRDM9, as well as in cells expressing Flag-ZCWPW2 alone. ( J ) Co-IP assays were performed in HEK293T cells co-transfected with HA- ZCWPW1 (W1) and Flag- ZCWPW2 (W2). (K and L) Co-IP assays were performed on human ( K ) and mouse ( L ) testis lysates to detect endogenous interactions between ZCWPW1 (W1) and ZCWPW2 (W2). ( M ) Schematic overview showing full-length ZCWPW2 ( W2 ) and ZCWPW1( W1 ), along with their domain-deletion variants lacking either the zf-CW domain (ΔC), the PWWP domain (ΔP), or both (ΔC-ΔP), were generated and were co-transfected in HEK293T cells. ( N ) Co-IP analysis showing the interaction between full-length ZCWPW1 (W1) and full-length or domain-deletion variants of ZCWPW2 (W2). ( O ) Co-IP analysis of ZCWPW2 containing the PWWP domain (W2-ΔC) and full-length or domain-deletion variants of ZCWPW1 (W1) in HEK293T cells. Data for ZCWPW1 ChIP-seq in HEK293T cells, either transfected alone or co-transfected with PRDM9, were obtained from the GEO repository under accession number GSE141516 . ChIP-seq data for PRDM9 in HEK293T cells were retrieved from GEO accession GSE99407 .

Article Snippet: Cells were treated with 20 mM lactate (MedChemEpress, HY-B2227) for 14 h. Full-length cDNAs of ZCWPW1, ZCWPW2, PRDM9 B , SYCP1, HSPA2, SYCE1, SPATA22, TEX11, MLH1, MSH2, LDHA , or MDC1 were synthesized and cloned into pcDNATM3.1(+) vectors with Flag, HA, or Myc tags by the commercial biotechnology companies PAIVIBIO (China) and TSINGKE (China).

Techniques: Binding Assay, Transfection, Expressing, Control, Co-Immunoprecipitation Assay, Generated, ChIP-sequencing

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: Synthetic scheme of mPEG-PLGA-PPA copolymer. PEG: poly(ethylene glycol); PLGA: poly( l -glutamic acid); PPA: poly( l -phenylalanine).

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques:

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: 1 H NMR of mPEG-poly(BLG)-PPA ( A ) and mPEG-PLGA-PPA ( B ) in DMSO-d 6.

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques:

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: FT-IR spectra of copolymer mPEG-PLGA-PPA (a) and mPEG-poly(BLG)-PPA (b).

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques:

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: Molecular weight of mPEG-PLGA-PPA copolymers.

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques: Molecular Weight

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: Critical micelle concentration (CMC) values of mPEG-PLGA-PPA copolymers.

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques: Concentration Assay

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: SEM micrographs of ( a ) mPEG 44 -PLGA 9 -PPA 14 showing maze pattern, ( b ) mPEG 44 -PLGA 9 -PPA 14 , ( c ) mPEG 44 -PLGA 20 -PPA 18 showing dendritic pattern and ( d ) mPEG 44 -PLGA 20 -PPA 18 .

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques:

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: Circular dichroism spectra of mPEG-PLGA-PPA micelles at different pH.

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques:

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: Secondary conformation of polymer micelles at different pH.

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques:

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: Drug loading profiles of mPEG-PLGA-PPA copolymers with different block ratios.

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques: Blocking Assay

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: Drug loading content (DLC) and drug loading efficiency (DLE) of Sorafenib (SFN)-loaded assembled micelles of different drug/carrier mass ratio.

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques:

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: In vitro drug release profiles of SFN loaded mPEG-PLGA-PPA micelles at pH 7.4.

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques: In Vitro

Journal: Pharmaceutics

Article Title: Polypeptides Micelles Composed of Methoxy-Poly(Ethylene Glycol)-Poly( l -Glutamic Acid)-Poly( l -Phenylalanine) Triblock Polymer for Sustained Drug Delivery

doi: 10.3390/pharmaceutics10040230

Figure Lengend Snippet: In vitro drug release profiles of SFN loaded mPEG-PLGA-PAA micelles.

Article Snippet: The critical micelle concentrations (CMC) of synthesized mPEG-PLGA-PPA was recorded on an Aminco Bowman Series 2 Luminescence Spectrometer (Spectrolab, Inc., Sylmar, CA, USA) by the fluorescence technique with a pyrene probe.

Techniques: In Vitro